You are interested in how gene expression is regulated by a…

Question Answered step-by-step You are interested in how gene expression is regulated by a… You are interested in how gene expression is regulated by a specific growth factor in cultured neuronal cells. To address the question of regulation of gene expression, you treat cells with growth factor and then use RNA Sequencing (RNASeq) to identify transcripts that show altered levels when you compare samples prior to treatment (Time=Oh) to those following a 3-hour (Time =3h) treatment with the growth factor. Your approach identifies one interesting transcript, NeuronQU, that shows a significant increase in response to treatment with growth factor.A) Briefly describe RNASeq. Mention another method that could be employed to identify changes in NeuronQU transcript levels. What are advantages and disadvantages of each?B) Your collaborator has worked out the details of the growth factor signaling pathway, including the phosphorylation of certain transcription factors. Describe an experiment to determine which of these transcription factors are present at the NeuronQU promoter in the presence of growth factor.C) Describe an experiment to quantitatively determine the affinity of the direct interaction of a transcription factor with NeuronQU promoter DNA.D) You suspect NeuronQU activation may involve chromatin remodeling. Describe one experiment to test this, and your expected results (i.e. what is the nucleosome positioning at an active vs repressed promoter, and how might you test this). Biology Science Biochemistry BIOL 3611 Share QuestionEmailCopy link Comments (0)