Using this link:…
Question Answered step-by-step Using this link:… Using this link: https://remotelabs.asdlib.org/wp-content/uploads/2020/09/Fluorescence-spectroscopy-VirtualLab-English.docxGather up all the data you need in the simulator and answer these questions:A. (1) Compare the ?max (ex) with ?max (em). If there is a ?E between the ground state and the excited state, why is the excitation ?max different than the fluorescence emission ?max. What is this displacement called? How is the behavior in general regarding the absorption and emission spectra of fluorescence and phosphorescence?(2) Use the two data files created during this exercise to generate a graph showing each of the spectra collected on the same set of axes. Label each spectrum and indicate ?max (ex) and ?max (em).B. (1) Plot intensity vs RhB concentration for each ? (ex) (?max (ex), 525 nm or 558 nm). Obtain the three equations of the line.(2) Comment on the results obtained. Explain why it is more convenient to quantify by selecting the maximum absorption / emission wavelengths?C. (1) Look up the structure of the Quinine molecule. Please indicate why it fluoresces(2) What effect does the addition of sodium chloride have on the fluorescence of quinine? Make a list of compounds that might have a similar effect.(3) Construct a table with the QBS intensity for each NaCl concentration. Plot these values. Interpret the graph. Arts & Humanities Writing BIO PMS Share QuestionEmailCopy link Comments (0)
