Questio- Protein A is an enzyme that catalyzes a reaction with…

Question Answered step-by-step Questio- Protein A is an enzyme that catalyzes a reaction with… Questio- Protein A is an enzyme that catalyzes a reaction with substrate S. This conversion occurs in the binding site of A. We know that binding sites are typically hydrophobic and designed to exclude water. Suppose that S is a negatively charged molecule, and it interacts with an ionic Lys residue in this binding pocket.a) According to the integrated Coulomb’s law, the energy of an ionic bond is E = (3.3 x 10-8 kcal ×m/mol)(z1)(z2)/(D*r), where z is the integer charge value of the molecules involved, and r is a distance between two charged ions. The dielectric constant (D) is 78 in water and 4 in a hydrophobic pocket. Use this relationship to show how the presence of water in the binding pocket would affect this interaction versus if water were excluded.b) How would raising the pH to 12 affect protein A’s interaction with substrate S? Calculate the energy of the interaction using Coulomb’s law as given above.  Biology Science Biochemistry CHEM 153A UCDAVIS Share QuestionEmailCopy link Comments (0)