Laboratory Assignment #4 Mannitol Salt Agar Plates – 25 points…
Question Laboratory Assignment #4 Mannitol Salt Agar Plates – 25 points… Laboratory Assignment #4Mannitol Salt Agar Plates – 25 points Today you will be growing bacteria from your hands onto mannitol salt agar (MSA) plates. This type of agar is considered to be both selective media and differential media.Experimental ProcedureObtain a MSA plateDivide the plate into two halves by marking the bottom of the plate with a marker. On one side, inoculate your plate with your unwashed hand using a sterile swab.Wash your hands with soap for 60 secondsInoculate the other side of the plate with your washed hands using a sterile swab.Ensure your plate is properly labeled with your initials and the date.Incubate your plate (with the lid on the bottom) at 37°C. Please review the MSA Lab Lecture Power Point Questions:Describe the difference between selective and differential media (2 points). Describe in detail (in complete sentences) how MSA plates function to be both selective (4 points) and differential media (6 points)? Please use the image below to answer the following questions. Results:Do you have more or less growth in the unwashed portion of your plate (2 points)? Is this growth Gram positive or Gram negative bacteria (2 points)? Is the growth you observe fermenting mannitol, or non-fermenting mannitol (2 points)? Briefly describe what your plate looks like – both the colonies and the agar (2 points). Is using this media (MSA) useful for studying Gram negative bacteria (1 point)? What is another media you might employ to select for Gram negative bacterial growth (2 points)? Is this other media you chose to select for Gram negative growth selective, differential or both – explain (2 points)? Biology Science Microbiology BIO 120 Share QuestionEmailCopy link Comments (0)
