A. Preparation of the sample Add 1 g of oxalic acid to about 100 ml…
Question A. Preparation of the sample Add 1 g of oxalic acid to about 100 ml… A. Preparation of the sampleAdd 1 g of oxalic acid to about 100 ml of the juice in a beaker to stabilize the ascorbic acid. Filter. B. TitrationRinse the burette with a little 0.1N iodine solution; then fill the burette with the iodine solution. Be certain that there are no air bubbles in the tip or just above the stopcock of the burette. Read and record the initial volume of the solution.Measure 3 samples of 25 ml of the filtered fruit juice. Add 1 ml of 0.50% starch solution. The starch solution, which is used as the indicator, should be freshly prepared and should not be used after 24 hrs of refrigerated storage.Titrate with the iodine solution until a definite color change is observed. Sometimes, the color will fade on standing, so take the first time it changes as your endpoint. Record the volume of iodine used.NOTEOne milliliter of the iodine solution is equivalent to 0.30 mg of Vitamin C. Calculate the number of milligrams of vitamin C in the sample. INSTRUCTION:All final answers should be rounded to 4 significant figures.Divide the computations among all group members. The name of the member who did the computation must be written below the final answer. DATA AND RESULTS: Complete the table by filling up the required data and results based on the computations. Verdana 10 blue. Sample 1Sample 2Sample 3Amount of sample 35 mL35 mL35 mLInitial volume reading of I2 (mL)0 mL0.10 mL0.30 mLFinal volume reading of I2 (mL)0.15 mL0.47 mL0.58 mLVolume of iodine used (mL) Amount of vitamin C present, mg % Vitamin C COMPUTATIONS:Format:Handwritten (show all solutions and box the final answers), photographed clearly and pasted here. Take note of the format to avoid deductions. Biology Science Biochemistry Share QuestionEmailCopy link Comments (0)
