A specific regulator of sex-determination, known as TRA2A…

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Question Answered step-by-step A specific regulator of sex-determination, known as TRA2A… A specific regulator of sex-determination, known as TRA2A (transformer 2 alpha homolog), may be under positive selection in birds with high degrees of post-copulatory sexual selection (e.g. sperm competition). You have recently obtained TRA2A sequences from Indian peafowl (Pavo cristatus). The gene has 6 exons, 2 of which are of particular interest (exons 1 and 5). You sequenced TRA2A in 26 individuals and found 4 polymorphic sites in exon 1 and 11 in exon 5. A colleague that works on Congo peafowl (Afropavo congensis) sequenced TRA2A in 13 individuals. Comparing the TRA2A sequences from both peafowl species, you find that 11 sites differ in sequences from exon 1 and 10 sites differ within exon 5. You also have DNA sequences from ?PGK-1, a neutrally-evolving pseudogene. Comparing the sequences, you notice 22 sites in ?PGK-1 vary between P. cristatus and A. congensis. Within your P. cristatus samples, there are 7 variable sites in ?PGK-1. Use the Hudson-Kreitman-Aguade (HKA) test to determine if regions of TRA2A are undergoing positive selection. Discuss your conclusion. For calculations of X2 please use the critical value of 3.84. Biology Science Genetics BIO BIOC16H3 Share QuestionEmailCopy link Comments (0)