I’m having a really hard time trying to answer most of this…

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Question Answered step-by-step I’m having a really hard time trying to answer most of this… I’m having a really hard time trying to answer most of this question. PLEASE help me!questions are based on the article above 1. What is the OVERALL question, the authors were trying to address in this paper?2. What is a protein chimera?3. What are at least THREE of the different protein chimeras that the researchers created and used?4. What was the purpose of the authors utilizing a temperature block in their experiments? Explainyour answer in terms of the questions they were asking for the overall paper. 5. Provide the direct quote from the paper when the authors state why they did figure 2.6. Explain why scientifically, the authors had to prove similarity or difference between Figure 1 and Figure 2.7. Draw a picture of what AQP434 protein structure looks like. Draw another picture of what you think AQP343 would look like. Provide a key or label your drawing so it is obvious which parts belong to which parts. (Draw the protein color coded…. Maybe AQP4 parts blue and AQP3 parts green…. Or in morse code with dashes for AQP3 and dots for AQP4)8. What was the purpose of the experiment in Figure 4?9. Using the knowledge from this paper, what would you predict would happen if the authors made AQ343 and co-stained for AQ4.10. Create a sources cited list for this worksheet.Image transcription textOPEN ACCESS Freely available online @PLOS | ONEAquaporin-3 and Aquaporin-4 Are Sorted Differently andSeparately in the Trans-Golgi Network Eva C. Arns… Show more… Show moreImage transcription textTrans-Golgi Network Sorting of AQP3 and AQP4 AQP3/AQP4AQP3. AQP4 AQP3/AQP4 AQP3 5 AQP4 Figure 1. AQP3 andAQP4 are separated in post-Golgi carriers. Repres… Show more… Show moreImage transcription textTrans-Golgi Network Sorting of AQP3 and AQP4 A AQP /VSV-GAQP3 VSV-G AQP4 VSV-G Nucleus Nucleus + 5 AQP3/VSV-GAQP4/VSV-G AQP3 AQP4 VSV-G VSV-G C LDL-R… Show more… Show moreImage transcription textTrans-Golgi Network Sorting of AQP3 and AQP4 AQP434/AQP4AQP434 AQP4 1 W N Nucleus AQP434/AQP4 AQP434 AQP4Figure 4. AQP4 and AQP434 co-localize in post-Go… Show more… Show moreImage transcription textTrans-Golgi Network Sorting of AQP3 and AQP4 express AQP3at significant levels until they reach confluency [17], proteinsdestined for the same plasma membrane domain t… Show more… Show moreImage transcription textTrans-Golgi Network Sorting of AQP3 and AQP4AQP3/Transferrin AQP3 Transferrin AQP4/Transferrin AQP4Transferrin Nucleus Nucleus Nucleus AQP3/Transfe… Show more… Show moreImage transcription textTrans-Golgi Network Sorting of AQP3 and AQP4 only plasmamembrane levels of AQP3 increase [28]. The on collagen-coatedcoverslips and allowed to attach and spread for ind… Show more… Show moreImage transcription textTrans-Golgi Network Sorting of AQP3 and AQP4 anti-AQP4antibodies as follows: Cells were fixed for 10 minutes magnifiedregions from the TGN. Scale bars are 5 um; 0.5 um … Show more… Show moreImage transcription textTrans-Golgi Network Sorting of AQP3 and AQP4 ReferencesNejsum LN, Nelson WJ (2009) Epithelial cell surface polarity: theearly steps. 17. Matsuzaki T, Suzuki T, Takata K (20… Show more… Show more  Biology Science cell biology BIOL 350 Share QuestionEmailCopy link Comments (0)