1. The slope of a plot of protein-ligand absorbance versus…
Question Answered step-by-step 1. The slope of a plot of protein-ligand absorbance versus… 1. The slope of a plot of protein-ligand absorbance versus concentration in micrograms/ml is 0.0435. What is the concentration of a sample with absorbance = 0.35? 2. Explain what is the basis for regression methods such as use with lm and nls commands. 3.Why is the binding curve approach considered more accurate than the titration analysis, for determining pka values? 4. For an acid-base system, the fraction bound can be defined as: HA ? A- + H+ F = 1 – (fraction A- formed) In a titration, VA = volume of base addedVE = volume base added at the endpointCB = molarity of the base Vi = initial volume of the acid The fraction A- formed is simply the moles A- formed (as determined by the titration) divided by the maximum A – formed. In the class we took into account that HA dissociates. a.Show the expression for F we used to transform titration data to fraction bound – for a monoprotic system. b.What is the fraction bound VA = 5.00 ml, VE = 10.00, CB = 0.100 M, Vi = 25.0 ml, andpH = 2 Science Chemistry CHEMISTRY 243 Share QuestionEmailCopy link Comments (0)
